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IL-10 and IL-12 increased cell surface expression of the costimulatory molecules CD86 and CD40 and had a marginal effect on CD80. The proinflammatory cytokine tumor necrosis factor-alpha induced CD80 and CD86. In contrast, IL-10 and IL-12 inhibited DC-mediated antigen-specific T-cell proliferation and the up-regulation of HLA-DR and intercellular adhesion molecule-1. These effects were accompanied by a shift of cytokine production from IL-12 toward IL-10, but the majority of monocyte-derived DCs produced neither IL-12 nor IL-10. 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